| 质粒名称: | 5TEY (METTL14) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 101891 |
| 存储实验室: | Cheryl Arrowsmith |
|
载体骨架基本信息 |
|
| 载体名称: | pFBOH-LIC notag |
| 完整质粒大小: | 5876 bp |
| 载体类型: | Baculovirus expression |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | METTL14 / gi|34147341 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 951 bp |
| 启动子: | polyhedrin |
| 克隆方法: | Ligation Independent Cloning |
| 5’测序引物 | rpFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3') |
| 3’测序引物 | pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3') |
|
文献引用方法 |
|
| 材料和方法部分: | 5TEY (METTL14) was a gift from Cheryl Arrowsmith (Addgene plasmid # 101891 ; http://n2t.net/addgene:101891 ; RRID:Addgene_101891) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS 5TEY (METTL14) 5876 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Baculovirus expression for structure determination; may not be full
ORF.
ACCESSION .
VERSION .
KEYWORDS 5TEY (METTL14)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5876)
TITLE SGC Toronto Q2 Q3 2016
REFERENCE 2 (bases 1 to 5876)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5876
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..92
/gene="polh from Autographa californica"
/label=polyhedrin promoter
/note="promoter for the baculovirus polyhedrin gene"
primer_bind 17..34
/label=Polyhedrin-F
/note="Polyhedrin promoter, forward primer"
polyA_signal 1482..1616
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1519..1538)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1573..1592
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
mobile_element 1645..1810
/mobile_element_type="transposon:Tn7"
/note="mini-Tn7 element (left end of the Tn7 transposon)"
rep_origin 1994..2448
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2081..2100)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2290..2311
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 2474..2578
/gene="bla"
/label=AmpR promoter
CDS 2579..3439
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2797..2816)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3610..4198
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4099..4118
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 4352..4369
/label=L4440
/note="L4440 vector, forward primer"
mobile_element 4503..4727
/mobile_element_type="transposon:Tn7"
/note="mini-Tn7 element (right end of the Tn7 transposon)"
promoter complement(5497..5525)
/gene="intI1 (promoter lies within the coding sequence)"
/label=Pc promoter
/note="class 1 integron promoter"
ORIGIN
1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
121 tcgggcgcgg atctcggtcc gaaaaccatg gatagccgct tgcaggagat ccgggagcgg
181 cagaagttac ggcgacagct cctcgcgcag cagttgggag ctgaaagtgc cgacagcatt
241 ggtgccgtgt taaatagcaa agatgagcag agagaaattg ctgaaacaag agaaacttgc
301 agggcttcct atgatacctc tgctccaaat gcaaaacgta agtatctgga tgaaggagag
361 acagatgaag acaaaatgga agaatataag gatgaactag aaatgcaaca ggatgaagaa
421 aatttgccat atgaagaaga gatttacaaa gattctagta cttttcttaa gggaacacag
481 agcttaaatc cccataatga ttactgccaa cattttgtag acactggaca tagacctcag
541 aatttcatca gggatgtagg tttagctgac agatttgaag aatatcctaa actgagggag
601 ctcatcaggc taaaggatga gttaatagct aaatctaaca ctcctcccat gtacttacaa
661 gccgatatag aagcctttga catcagagaa ctaacaccca aatttgatgt gattcttctg
721 gaaccccctt tagaagaata ttacagagaa actggcatca ctgctaatga aaaatgctgg
781 acttgggatg atattatgaa gttagaaatt gatgagattg cagcacctcg atcatttatt
841 tttctctggt gtggttctgg ggaggggttg gaccttggaa gagtgtgttt acgaaaatgg
901 ggttacagaa gatgtgaaga tatttgttgg attaaaacca ataaaaacaa tcctgggaag
961 actaagactt tagatccaaa ggctgtcttt cagagaacaa aggaacactg cctcatgggg
1021 atcaaaggaa ctgtgaagcg tagcacagac ggggacttca ttcatgctaa tgttgacatt
1081 gacttaatta tcacagaaga acctgaaatt ggcaatatag aaaaacctgt agaaattttt
1141 catataattg agcatttttg tcttggtaga agacgccttc atctatttgg aagagatagt
1201 acaattcgac caggctggct cacagttgga ccaacgctta caaatagcaa ctacaatgca
1261 gaaacatatg catcctattt cagtgctcct aattcctact tgactggttg tacagaagaa
1321 attgagagac ttcgaccaaa atcgtgatga cgaagcttgt cgagaagtac tagaggatca
1381 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
1441 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
1501 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
1561 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat
1621 cactgatatc gcctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg
1681 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
1741 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
1801 tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact
1861 ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga aaatttttct
1921 gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat ttgcagcctg
1981 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
2041 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
2101 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
2161 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
2221 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
2281 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
2341 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
2401 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
2461 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
2521 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
2581 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
2641 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
2701 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
2761 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
2821 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
2881 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
2941 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
3001 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
3061 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
3121 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
3181 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
3241 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
3301 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
3361 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
3421 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
3481 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
3541 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
3601 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
3661 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
3721 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
3781 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
3841 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
3901 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
3961 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
4021 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
4081 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
4141 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
4201 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
4261 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
4321 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
4381 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca
4441 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg
4501 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
4561 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
4621 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
4681 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac
4741 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg
4801 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag
4861 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc
4921 gcgttggcct catgcttgag cagattgatg agcgcggtgg caatgccctg cctccggtgc
4981 tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc
5041 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg
5101 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag
5161 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact
5221 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt
5281 ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
5341 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca
5401 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
5461 gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat
5521 gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc
5581 agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc
5641 acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat
5701 ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg
5761 accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc
5821 caggactcta gctatagttc tagtggttgg ctacgtatac tccggaatat taatag
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：METTL3
- 下一篇：没有了
